| Product name: | Human NEFL(Neurofilament, Light Polypeptide) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 46.4 pg/mL |
| Standard: | 10000 pg/mL |
| Range: | 156.25-10000 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Neuro science; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NEFL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NEFL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NEFL in the samples is then determined by comparing the OD of the samples to the standard curve. |