Detection instrument: Microplate reader(500-530 nm,optimum wavelength: 520 nm)
Detection method: Colorimetric method
Detection principle:
Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.
Performance characteristics
| Synonyms | ALP |
| Sample type | Serum,plasma,tissue,cells |
| Sensitivity | 0.13 King unit/100 mL |
| Detection range | 0.13-50 King unit/100 mL |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 30 min |
| Precision | Average inter-assay CV: 8.500%Average intra-assay CV: 5.100% |
| Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 12 months |