| Size | 100 ug |
|---|---|
| Applications | ELISA, WB |
| Expression | SF9 cells |
| Purification | Column chromatography |
| Target | Marburg Virus |
Description: Mature, recombinant, His-tagged Angola marburgvirus Glycoprotein minus the transmembrane domain (MARV-Angola rGPΔTM) is supplied as purified protein. MARVAngola rGPΔTM is produced in Sf9 insect cells using baculovirus for expression and is purified by FPLC.
Storage: 2-3 weeks at -20◦ C, -80◦ C long term Size: 100 µg of protein supplied in PBS (supplemented with glycerol, arginine and glutamic acid) at a concentration of 0.334 mg/mL.
The theoretical molecular weight of the protein is ~60 kDa including the His-tag, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.
Relevance: Recombinant glycoprotein provides a means for
antibody development, control protein for testing, and a tool to
enhance research.
Western Blot: Quality control testing demonstrates strong
detection of GP null and GP2 under reduced conditions.