Platinum-E Retroviral Packaging Cell Line, Ecotropic,1 vial
Product details
Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells.
The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.
High Retroviral Yields with Plat-E cells. NIH3T3 cells and mouse ProB Ba/F3 cells.
High Retroviral Yields with Plat-E cells. NIH3T3 cells were infected with GFP retrovirus supernatant produced in Plat-E cells after transfection with pMX-GFP.